Human MDC ELISA Kit (ab223866) (2024)

Overview

• Product name

  Human MDC ELISA Kit
  See all MDC kits

• Detection method

  Colorimetric

• Precision

  Intra-assay

  Sample	n	Mean	SD	CV%
  Serum	5			2.8%

  Inter-assay

  Sample	n	Mean	SD	CV%
  Serum	3			2.1%

• Sample type

  Cell culture supernatant, Serum, Hep Plasma, EDTA Plasma, Cit plasma

• Assay type

  Sandwich (quantitative)

• Sensitivity

  1.3 pg/ml

• Range

  3.1 pg/ml - 200 pg/ml

• Recovery

  Sample specific recovery

  Sample type	Average %	Range
  Cell culture supernatant	111	102% - 121%
  Serum	108	103% - 115%
  Cell culture media	100	98% - 102%
  Hep Plasma	78	60% - 88%
  EDTA Plasma	89	79% - 106%
  Cit plasma	118	111% - 131%

• Assay time

  1h 30m

• Assay duration

  One step assay

  See Also

  Anti-MDC antibody (ab9847) | AbcamPermit Price Adjustments 2025Maladie débilitante chronique - inspection.canada.ca

• Species reactivity

  Reacts with: Human, Rhesus monkey
  Does not react with: Cow

• Product overview

  Human MDC ELISA Kit (ab223866) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MDC protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MDC with 1.3 pg/ml sensitivity.

  
  

  SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

  
  

  - Single-wash protocol reduces assay time to 90 minutes or less
  - High sensitivity, specificity and reproducibility from superior antibodies
  - Fully validated in biological samples
  - 96-wells plate breakable into 12 x 8 wells strips

  
  

  A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

• Notes

  Macrophage-derived chemokine (MDC), also known as C-C motif chemokine 22 (CCL22), is a secreted cytokine and member of the intercrine beta (chemokine CC) family. It is highly expressed in macrophage and in monocyte-derived dendritic cells. MDC may play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. MDC is a chemotactic for monocytes, dendritic cells and natural killer cells and potent chemoattractant for chronically activated T-lymphocytes.

• Platform

  Pre-coated microplate (12 x 8 well strips)

Properties

• Storage instructions

  Store at +4°C. Please refer to protocols.

• Components	1 x 96 tests
  10X Human MDC Capture Antibody	1 x 600µl
  10X Human MDC Detector Antibody	1 x 600µl
  Human MDC Lyophilized Recombinant Protein	2 vials
  Antibody Diluent 4BI	1 x 6ml
  10X Wash Buffer PT (ab206977)	1 x 20ml
  TMBDevelopment Solution	1 x 12ml
  Stop Solution	1 x 12ml
  Sample Diluent NS (ab193972)	1 x 50ml
  SimpleStep Pre-Coated 96-Well Microplate (ab206978)	1 unit
  Plate Seal	1 unit

• Research areas

  • Immunology
  • Innate Immunity
  • Chemokines
  • Beta Chemokines (CC)

  • Neuroscience
  • Processes

• Function

  May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.

• Tissue specificity

  Highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. Also found in lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression in lung and spleen. Very weak expression in small intestine. In lymph node expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+). Expressed in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis.

• Sequence similarities

  Belongs to the intercrine beta (chemokine CC) family.

• Post-translational
  modifications

  The N-terminal processed forms MDC(3-69), MDC(5-69) and MDC(7-69) are produced by proteolytic cleavage after secretion from monocyte derived dendrocytes.

• Cellular localization

  Secreted.

• Information by UniProt

• Alternative names

  • A 152E5.1
  • ABCD 1
  • ABCD1

  see all

• Database links

  • Entrez Gene: 6367 Human
  • Omim: 602957 Human
  • SwissProt: O00626 Human
  • Unigene: 534347 Human

• Human MDC standard curve comparison data

  Standard curve comparison between human MDC SimpleStep ELISA^®kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

• Example of human MDC standard curve in Sample Diluent NS.

  Background-subtracted data values (mean +/- SD) are graphed.

• Interpolated concentrations of native MDC in human serum, plasma and cell culture supernatant samples.

  The concentrations of MDC were measured in duplicates, interpolated from the MDC standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (citrate) 25%, plasma (heparin) 45%, plasma (EDTA) 25%, and 2 days 1.5% PHA-treated PBMC supernatant 4%. The interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean MDC concentration in neat sample was determined to be 678 pg/mL in serum, 844 pg/mL in plasma (citrate), 435 pg/mL in plasma (heparin), 781 pg/ml in plasma (EDTA), and 4352 pg/ml in PHA-treated PBMC supernatant.

• Serum from ten individual healthy human male donors was measured in duplicate.

  Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean MDC concentration was determined to be 698 pg/mL with a range of 376 – 967 pg/mL.

• Comparison of MDC in unstimulated and PHA stimulated PBMC cell supernatants.

  PBMC cells were cultured for 46 hours in the presence or absence of 1.5% PHA. The concentrations of MDC were measured in three dilutions of supernatant samples in duplicates and interpolated from the MDC standard curve. The interpolated values are plotted (mean +/- SD, n=3). The mean MDC concentration was determined to be 4519 pg/mL in neat PHA stimulated PBMC cell culture supernatant and 27 pg/mL in neat unstimulated PBMC cell culture supernatant.

• Serial dilutions of recombinant human 67 amino acid long MDC

  Serial dilutions of recombinant human 67 amino acid long MDC (aa27-93) were prepared and assayed in parallel with 69 amino acid long MDC (aa25-93), the standard of this kit.

• Sandwich ELISA - Human MDC ELISA Kit (ab223866)

  SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

• Sandwich ELISA - Human MDC ELISA Kit (ab223866)

  To learn more about the advantages of recombinant antibodies see here.

• Sandwich ELISA - Human MDC ELISA Kit (ab223866)

  To learn more about the advantages of SimpleStep ELISA^® kits see here.

• Protocol Booklet

Click here to view the general protocols

• SDS download

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• Datasheet download

  Download

ab223866 has been referenced in 1 publication.

• Harms RZ et al. Confirmation and Identification of Biomarkers Implicating Environmental Triggers in the Pathogenesis of Type 1 Diabetes. Front Immunol 11:1922 (2020). PubMed: 33042112