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Abpromise
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Overview
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Product name
Anti-MDC antibody
See all MDC primary antibodies -
Description
Rabbit polyclonal to MDC
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Host species
Rabbit
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Tested applications
Suitable for: Sandwich ELISA, WBmore details
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Species reactivity
Reacts with: Human
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Immunogen
Recombinant full length protein corresponding to Human MDC. Highly pure (>98%) recombinant hMDC(human Macrophage-Derived Chemokine)
Database link: O00626 -
Positive control
- Recombinant human MDC protein (ab9848) can be used as a positive control in WB.
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General notes
This product is no longer batch tested in IHC, for an IHC validated antibody please seeab124768
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage buffer
No preservative, sterile filtered
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Concentration information loading... -
Purity
Immunogen affinity purified
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Clonality
Polyclonal
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Isotype
unknown
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Light chain type
unknown
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Research areas
- Immunology
- Innate Immunity
- Chemokines
- Beta Chemokines (CC)
- Neuroscience
- Processes
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9847 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use a concentration of 0.5 - 2 µg/ml. | |
| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 11 kDa. |
| Notes |
|---|
| Sandwich ELISA |
| WB |
Target
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Function
May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active.
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Tissue specificity
Highly expressed in macrophage and in monocyte-derived dendritic cells, and thymus. Also found in lymph node, appendix, activated monocytes, resting and activated macrophages. Lower expression in lung and spleen. Very weak expression in small intestine. In lymph node expressed in a mature subset of Langerhans' cells (CD1a+ and CD83+). Expressed in Langerhans' cell histiocytosis but not in dermatopathic lymphadenopathy. Expressed in atopic dermatitis, allergic contact dermatitis skin, and psoriasis, in both the epidermis and dermis.
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Sequence similarities
Belongs to the intercrine beta (chemokine CC) family.
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Post-translational
modificationsThe N-terminal processed forms MDC(3-69), MDC(5-69) and MDC(7-69) are produced by proteolytic cleavage after secretion from monocyte derived dendrocytes.
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Cellular localization
Secreted.
- Information by UniProt
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Database links
- Entrez Gene: 6367 Human
- Omim: 602957 Human
- SwissProt: O00626 Human
- Unigene: 534347 Human
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Alternative names
- A 152E5.1 antibody
- ABCD 1 antibody
- ABCD1 antibody
see all
Datasheets and documents
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Datasheet download
Download
References (9)
Publishing research using ab9847? Please let us know so that we can cite the reference in this datasheet.
ab9847 has been referenced in 9 publications.
- Liang H & Peng J LncRNA HOTAIR promotes proliferation, invasion and migration in NSCLC cells via the CCL22 signaling pathway. PLoS One 17:e0263997 (2022). PubMed: 35176085
- Jiang Z et al. MicroRNA-23a-3p influences the molecular mechanism of gastric cancer cells via CCL22/PI3K/Akt axis. Bioengineered 12:11277-11287 (2021). PubMed: 34874224
- Wang L et al. Anti-CCL22 increases regulatory T cells in CD4+ T cells of rheumatoid arthritis patients via STAT5 pathway. Exp Ther Med 19:2127-2132 (2020). PubMed: 32104275
- Zhang K et al. HBV promotes the recruitment of IL-17 secreting T cells via chemokines CCL22 and CCL17. Liver Int 40:1327-1338 (2020). PubMed: 32187823
- Wu S et al. C-C motif chemokine 22 predicts postoperative prognosis and adjuvant chemotherapeutic benefits in patients with stage II/III gastric cancer. Oncoimmunology 7:e1433517 (2018). PubMed: 29872564
- Liang D et al. Activated STING enhances Tregs infiltration in the HPV-related carcinogenesis of tongue squamous cells via the c-jun/CCL22 signal. Biochim Biophys Acta 1852:2494-503 (2015). PubMed: 26303640
- Döring C et al. A novel immunohistochemical classifier to distinguish Hodgkin lymphoma from ALK anaplastic large cell lymphoma. Mod Pathol N/A:N/A (2014). PubMed: 24633193
- Li YQ et al. Tumor Secretion of CCL22 Activates Intratumoral Treg Infiltration and Is Independent Prognostic Predictor of Breast Cancer. PLoS One 8:e76379 (2013). IHC-P ; Human . PubMed: 24124553
- Qin XJ et al. CCL22 recruits CD4-positive CD25-positive regulatory T cells into malignant pleural effusion. Clin Cancer Res 15:2231-7 (2009). IHC-P ; Human . PubMed: 19318474
Customer reviews and Q&As
